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Direct layer-by-layer (LbL) assembly of cationic, small-molecule antibacterial bioactives with water-soluble, ionic polyphosphazenes (PPzs) containing trifluoroethoxy and carboxy substitients is reported. First, influence of PPzs hydrophobicity and antibiotic charge density on LbL assembly was studied via evolution of dry film thickness. We found that the use of fluorinated PPz polyelectrolytes enhanced ionic pairing within LbL coatings, and that increasing charge density of small molecules increased antibiotic uptake. This strategy was successful even in the case of gentamicin, a hydrophilic, small antibiotic with only 3 to 4 positive charges at pH 7.5. Confirmation of antibiotic presence in films was demonstrated via x-ray photoelectron spectroscopy. Importantly, LbL films of fluorinated PPz polyelectrolytes retained antibiotics in physiological conditions due to the enhanced hydrophobic interactions. In contrast, LbL films of non-fluorinated PPzs released antibiotics at low pH and in the presence of salt following the charge renormalization argument. The potential of these coatings with a biomedically relevant bacterial strain, Staphylococcus aureus, is discussed. 

A fundamental assumption in biological anthropology is that living individuals will present with different growth than non‐survivors of the same population. The aim is to address the question of whether growth and development data of non‐survivors are reflective of the biological consequences of selective mortality and/or stress.

The study compares dental development and skeletal growth collected from radiographic images of contemporary samples of living and deceased individuals from the United States (birth to 20 years) and South Africa (birth to 12 years). Further evaluation of deceased individuals is used to explore differential patterns among manners of death (MOD).

Results do not show any significant differences in skeletal growth or dental development between living and deceased individuals. However, in the South African deceased sample the youngest individuals exhibited substantially smaller diaphyseal lengths than the living sample, but by 2 years of age the differences were negligible. In the US sample, neither significant nor substantial differences were found in dental development or diaphyseal length according to MOD and age (>2 years of age), though some long bones in individuals <2 years of age did show significant differences. No significant differences were noted in diaphyseal length according to MOD and age in the SA sample.

The current findings refute the idea that contemporary deceased and living individuals would present with differential growth and development patterns through all of ontogeny as well as the assumptions linking short stature, poor environments, and MOD.

 

Abstract In particle collider experiments, elementary particle interactions with large momentum transfer produce quarks and gluons (known as partons) whose evolution is governed by the strong force, as described by the theory of quantum chromodynamics (QCD) 1 . These partons subsequently emit further partons in a process that can be described as a parton shower 2 , which culminates in the formation of detectable hadrons. Studying the pattern of the parton shower is one of the key experimental tools for testing QCD. This pattern is expected to depend on the mass of the initiating parton, through a phenomenon known as the dead-cone effect, which predicts a suppression of the gluon spectrum emitted by a heavy quark of mass m Q and energy E , within a cone of angular size m Q / E around the emitter 3 . Previously, a direct observation of the dead-cone effect in QCD had not been possible, owing to the challenge of reconstructing the cascading quarks and gluons from the experimentally accessible hadrons. We report the direct observation of the QCD dead cone by using new iterative declustering techniques 4,5 to reconstruct the parton shower of charm quarks. This result confirms a fundamental feature of QCD. Furthermore, the measurement of a dead-cone angle constitutes a direct experimental observation of the non-zero mass of the charm quark, which is a fundamental constant in the standard model of particle physics.